Part:BBa_K2407003:Design

CYC1 terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

we use overlap to link promoter, FLO1 gene and terminator so we need to learn to design the overlap forward primer. We use digestion and connection to add this whole part to vector. So we meed to add restriction site and protecting bases when we design the reverse primer.

Source

use PCR to amplify the part from a plasmid